A Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method
نویسندگان
چکیده
منابع مشابه
a nested-splicing by overlap extension pcr improves specificity of this standard method
background: splicing by overlap extension (soe) pcr is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein’s structure and function.objectives: we introduced a nested-soe-pcr (n –soe-pcr) in order to increase the specificity and generating mutations in a gene by soe-pcr. materials and methods: genomic dna from bacillus thermoca...
متن کاملA Nested-Splicing by Overlap Extension PCR Improves Specificity of this Standard Method.
BACKGROUND Splicing by overlap extension (SOE) PCR is used to create mutation in the coding sequence of an enzyme in order to study the role of specific residues in protein's structure and function. OBJECTIVES We introduced a nested-SOE-PCR (N -SOE-PCR) in order to increase the specificity and generating mutations in a gene by SOE-PCR. MATERIALS AND METHODS Genomic DNA from Bacillus thermoc...
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The combined overlap extension PCR (COE-PCR) method developed in this work combines the strengths of the overlap extension PCR (OE-PCR) method with the speed and ease of the asymmetrical overlap extension (AOE-PCR) method. This combined method allows up to 6 base pairs to be mutated at a time and requires a total of 40-45 PCR cycles. A total of eight mutagenesis experiments were successfully ca...
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ژورنال
عنوان ژورنال: Iranian Journal of Biotechnology
سال: 2015
ISSN: 1728-3043,2322-2921
DOI: 10.15171/ijb.1090